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Alternative methodologies for genotyping polymorphisms in the CAST and CAPN1 genes in beef cattle R. Bras. Zootec.
Blecha,Isabella Maiumi Zaidan; Sousa,Isadora Inácio; Ferreira,Anna Beatriz Robottom; Feijó,Gelson Luis Dias; Torres Júnior,Roberto Augusto de Almeida; Siqueira,Fabiane.
ABSTRACT The objectives of this study were to genotype single nucleotide polymorphisms (SNP) AF159246:g.2959A>G (CAST/DdeI) and AF248054.2:g.6545C>T (CAPN4751) in beef cattle by PCR-RFLP (Polymerase Chain Reaction - Restriction Fragment Length Polymorphism), using the restriction enzyme DdeI for both SNP, and describe the use of these genotyping methodologies for the first time. For the SNP located in the CAST gene, new primers were designed, and for the SNP of the CAPN1 gene, the same primers previously described in the literature were used. Bonsmara, Caracu, Senepol, Nelore, and Angus bulls were chosen from among the most used bulls in breeding programs according to their genealogy and the lowest possible degree of parentage between them to ensure...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Animal breeding; Marker-assisted selection; PCR-RFLP; SNP; Tenderness.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982019000100311
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Avaliação do efeito do tempo de desossa sobre a qualidade da carne de peito de matrizes pesadas de descarte - DOI: 10.4025/actascianimsci.v32i1.6128 Animal Sciences
Sanfelice, Cristiane; Unesp; Mendes, Ariel Antonio; Komiyama, Claudia Marie; Cañizares, Marleide Costa; Rodrigues, Luciana; Cañizares, Gil Ignácio Lara.
Este trabalho teve por objetivo avaliar o efeito do tempo de desossa da carne de matrizes pesadas, para destinar esta carne ao possível consumo “in natura”. Portanto, foram coletados 80 peitos com osso em abatedouro comercial. No Laboratório de Qualidade de Carne da FMVZ/Unesp, Campus de Botucatu, Estado de São Paulo, os peitos foram desossados nos tempos zero (imediatamente após o resfriamento), 4, 8 e 12h “post-mortem”, sendo utilizados 20 peitos por tratamento. Com 24h “post-mortem”, foram avaliados os parâmetros: pH, cor (L*, a* e b*), perda de peso por cocção (PPC), capacidade de retenção de água (CRA), força de cisalhamento (FC), índice de fragmentação miofibrilar (MFI) e estrutura morfológica da fibra muscular (diâmetro da fibra e número de fibras...
Tipo: Análise laboratorial Palavras-chave: 5.04.00.00-2 fibra muscular; Galinha de descarte; Maciez 5.04.05.00-4 muscle fiber; Spent hen; Tenderness.
Ano: 2009 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/6128
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Biochemical and Utralmicroscopic Evaluation of Myofibril Proteins and Collagen during Ageing in Broiler Chicken PSE (Pale, Sof, Exudative) Meat BABT
Marchi,Denis Fabrício; Beteto,Flávia Maria; Almeida,Gleice Rocha dos Santos; Soares,Adriana Lourenço; Hernandez-Blazquez,Francisco Javier; Ida,Elza Iouko; Shimokomaki,Massami.
Abstract The aim of this work was to study the myofibril proteins and collagen fraction changes in broiler chickens PSE (pale, soft, exudative) meat during ageing and their relationship to meat quality. The results presented an increase of myofibril proteins and collagen solubility promoted by the enhanced proteases activities during storage. Ultramicroscopically, the PSE meat samples revealed intracellularly a sarcomere super contraction and lacunas within the A and I bands while Z-lines appeared very dense and fragmented in comparison to normal samples. This observation was noticed already at 4h storage while extracellularly collagen fibrils decreased visually within the endomysium only after 24h of conditioning. These results influenced the quality as...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cooking loss; Tenderness; Collagen content; Myofibrillar Fragmentation Index.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100503
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Carcass and meat traits of feedlot Nellore bulls fed different dietary energy sources R. Bras. Zootec.
Maia Filho,Geraldo Helber Batista; Barbosa,Fabiano Alvim; Emerick,Lucas Luz; Souza,Rafahel Carvalho; Figueiredo,Tadeu Chaves; Azevedo,Henrique de Oliveira; Cavalcanti,Luigi Francis Lima; Mandarino,Raphael Amazonas.
ABSTRACT The objective of this experimental study was to evaluate the effects of different dietary energy sources on the carcass and meat traits of feedlot Nellore bulls. A total of 48 Nellore bulls at 32 months of age with 354.35±19.56 kg body weight were studied for 85 days. The bulls were allotted to four groups of 12 individuals: Whole corn grain - diet based on whole corn grain; Ground corn - diet based on ground corn grain; Citrus pulp - diet based on citrus pulp; and Steam-flaked corn - diet based on steam-flaked corn. Diets did not influence carcass quality or meat quality. Average values for fat depth, rib eye area, shear force, total cooking losses, and carcass yield were 4.97 mm, 74.71 cm2, 5.85 kgf, 26.82%, and 52.0%, respectively. Based on the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fat; High-concentrate diets; Intake; Rib eye area; Tenderness.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982016000500265
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Carcass and meet characteristics of very young Angus x Nelore steers in the Agreste Potiguar region Rev. Ciênc. Agron.
Façanha,Debora Andréa Evangelista; Leite,Jacinara Hody Gurgel Morais; Queiroga,Rita de Cássia Ramos Egypto; Costa,Roberto Germano; Garruti,Déborah dos Santos; Silva,Tâmara Lúcia dos Santos.
This work aimed to evaluate the quantitative and qualitative characteristics of the carcass and meat of very young steers, ½ Red Angus x Nelore (NEL) and ¾ Red Angus x Nelore (RED). Fifty males were used, 25 from each genetic group, fed in feedlots from weaning (7 months) until reaching the age for slaughter (15 months). A difference was observed between the genetic groups for gains at weaning (158.57 kg NEL and 181.60 kg RED) but the weight at slaughter showed no statistical differences (412.33 kg NEL and 426.53 kg RED). Cold and hot carcass yield was not affected by the genetic group, with NEL bovines showing a yield of 50.49% and 52.55% and the RED of 50.91% and 52.89% respectively. The percentage of muscle (55% NEL and 53% RED), fat (26% NEL and 27%...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Composition of the carcass; Feedlot; Thickness of fat; Tenderness.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902014000300024
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Carcass characteristics and meat quality of Aberdeen Angus steers finished on different pastures R. Bras. Zootec.
Devincenzi,Thais; Nabinger,Carlos; Cardoso,Fernando Flores; Nalério,Élen Silveira; Carassai,Igor Justin; Fedrigo,Jean Kássio; Tarouco,Jaime Urdapilleta; Cardoso,Leandro Lunardini.
The present study was conducted to assess carcass features, physicochemical and sensory parameters of meat from steers finished on three types of pastures: natural pasture; natural pasture improved, fertilized and oversown with winter species; and annual summer grassland. The experiment was conducted from December 14, 2009 to November 30, 2010, with treatments distributed in a completely randomized design with a different number of replicates. Animals were used as experimental units. Experimental animals were Aberdeen Angus steers with twenty months of initial age and 354±27.4 kg of live weight, on average. The highest average daily gains were obtained for the annual summer grassland. There was no effect of treatments on carcass conformation. The highest...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Average daily gain; Fertilized natural pasture; Sensory panel; Sorghum pasture; Tenderness.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012000400030
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Carcass physical composition and meat quality of holstein calves, terminated in different finishing systems and slaughter weights Ciência e Agrotecnologia
Santos,Priscila Vincenzi dos; Paris,Wagner; Menezes,Luís Fernando Glasenapp de; Vonz,Douglas; Silveira,Magali Floriano da; Tubin,Jessica.
The Brazil sacrifices many dairy calves at birth, which can become a very serious problem merchandising. The objective of this experiment was to evaluate the physical carcass composition and meat quality characteristics of Holstein calves in feedlot or pasture with supplementation, slaughtered at 140, 180, 220 or 260 kg body weight. In the early termination calves had on average 57 kg and 58 days of age. The confined animals were fed forage (corn) and concentrated, in the ratio of 40:60 and concentrated pasture supplemented with 1% body weight. The experimental design was completely randomized in a factorial 2 x 4 (two feeding systems x four slaughter weights). It was observed a significant interaction between slaughter weight and finishing system only for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fat; Tenderness; Muscle; Marbling; Veal.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542013000500008
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Effect of energy concentration in the diets on sensorial and chemical parameters of Morada Nova, Santa Inez and Santa Inez x Dorper lamb meat R. Bras. Zootec.
Batista,Ana Sancha Malveira; Costa,Roberto Germano; Garruti,Déborah dos Santos; Madruga,Marta Suely; Queiroga,Rita de Cássia Ramos do Egypto; Araújo Filho,José Teodorico de.
The objective of this study was to investigate the influence of genotype and the energy concentration in the diet on the sensorial and chemical quality of lamb meat. It was evaluated samples from 18 Morada Nova animals, 18 Santa Inez animals, and 18 Santa Inez x Dorper crossbred animals, totalizing 54 animals. The animals were kept with two diets, with energy concentrations of 10.46 and 12.56 MJ ME/kg, respectively, and slaughtered at 30 kg average weight. It was determined contents of protein, moisture, fat and ash, as well as cooking loss, water-holding capacity, shear force, and sensorial attributes of flavour, firmness and juiciness of the meat. Genotype influenced the chemical composition of lamb meat because animals of Morada Nova breed presented the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Flavour; Juiciness; Tenderness.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982010000900021
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Hanging the beef carcass by the forequarter to improve tenderness of the Longissimus dorsi and Biceps femoris muscles Scientia Agricola
Luchiari Filho,Albino; Macedo,Renato Prates; Pereira,Angélica Simone Cravo; Silva,Saulo da Luz e; Leme,Paulo Roberto; Feitosa,Guilherme.
Hanging beef carcasses in different configurations in the cooler affect some carcass muscle tenderness. Forty Nellore steer carcasses (ten per day) were chosen at random in a federally inspected slaughter plant and hanged alternate left and right sides either in the traditional way by the hindquarter (HQ) or by the forequarter (FQ) also called "tenderbife". Carcasses were selected from steers up to 30 months old and had an average hot carcass weight of 244.1 kg. These carcasses were chilled for 48 hours, when samples from the Longissimus dorsi (LD) at the 12th rib and the Biceps femoris (BF) at the P8 site were removed, kept under refrigeration (0-2ºC) for five days and frozen for future analysis. The temperature of the LD after 24 hours taken at the 12th...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Beef cattle; Carcass suspension; Meat quality; Tenderness.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162005000500013
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Levels of feed supplementation on the qualitative aspects of meat from crossbred goats finished on caatinga Rev. Ciênc. Agron.
Silva,Daniel Cézar da; Guim,Adriana; Santos,Gladston Rafael de Arruda; Maciel,Maria Inês Sucupira; Soares,Luciana Felizardo Pereira.
ABSTRACTThe effect was evaluated of feed supplementation (0.0, 0.4, 0.8, and 1.2% of body weight) on the qualitative aspects of goat meat (moisture, ash, fat, protein, colouring using the CIE (L*, a* and b *) system, pH, cooking loss and shear force), and also on the sensory attributes of goat aroma, off-aroma, colour, texture, tenderness, flavour, off-flavour, juiciness and overall appearance. A total of 32 crossbred Anglo-Nubian goats were used, organised into randomised blocks and finished on a pasture of caatinga. There was a quadratic effect (p<0.05) on the levels of ash and protein, with a maximum of 1.06 and 20.44 g/100 g respectively. A positive linear affect (p<0.05) was seen on fat levels, with an increase of 0.52 g/100 g. There was a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Meat colouring; Feeding strategy; Native pasture; Flavour; Tenderness.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902015000400855
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Meat quality of suckling goat raised in differents feeding systems Animal Sciences
Santos, Norivaldo Lima; Sousa, Wandrick Hauss de; Gomes, Maria das Graças Cunha; Batista, Ana Sancha Malveira; Ramos, João Paulo de Farias; Cartaxo, Felipe Queiroga; Lira, Aianne Batista; Cavalcante, Iara Tamires Rodrigues.
The objective of this work was to evaluate the effect of three breeding systems on the organoleptic quality and the physico-chemical composition of kids goat meat. Were used thirty newborn baby goats of 3.35 kg ± 0.65 kg crossed Parda Alpina x Undefined Race (SPRD) submitted to three breeding systems: Traditional system-TS; Intensive Feeding System without Concentrate- IS and Intensive Feeding System with Concentrate- IS+C. When the goats reached 12 kg, the animals were slaughtered and chemical and physical analyzes were performed in the evaluation of the meat and sensorial. The experimental design was completely randomized, submitted to analysis of variance and compared by the Tukey test and Ryan-Einot-Gabriel-Welsh at 5%. All analyzed variables were also...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Flavour; Lean meat; Kids; Tenderness.
Ano: 2020 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/46547
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Ostrich (Struthio camellus) carcass yield and meat quality parameters Rev. Bras. Ciênc. Avic.
Balog,A; Almeida Paz,ICL.
This article aimed at compiling recent studies on the main factors that influence ostrich meat quality and carcass yield. Few articles investigated the effect of subspecies, which generally are not even mentioned. There are important dietary effects, particularly those caused by dietary protein to energy ratio. Rigor mortis follow-up studies showed that there are no losses in meat quality when carcasses are hot-deboned. Age at slaughter influences some meat quality traits, such as tenderness and lipid content. Few effects of gender have been observed, and at the same age at slaughter, both male and female present the same meat quality traits.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Carcass yield; Meat quality; Ostrich production; Tenderness.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2007000400002
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Qualitative-feed-restricted heavy swine: meat quality and morpho-histochemical characteristics of muscle fibers BABT
Fraga,Alessandro Luís; Thomaz,Maria Cristina; Kronka,Rodolfo Nascimento; Budiño,Fábio Enrique Lemos; Huaynate,Rizal Alcides Robles; Scandolera,Antônio João; Ruiz,Urbano dos Santos; D'Angelis,Flora Helena de Freitas.
To evaluate the effect of different levels of qualitative feed restriction (0, 5, 10, 15 and 20%) on pork quality and muscle morpho-histochemical characteristics, 60 castrated male swines were used. Ten animals were slaughtered at 89 kg BW. Other 50 pigs were fed experimental diets and slaughtered at 128 kg BW. Qualitative restriction increased pH45, and water holding capacity, and reduced the redness, yellowness and cholesterol concentrations of Longissimus lumborum. Quadratic tendency for oleic acid contents of loin and fiber cross-section area of Multifidus dorsi (P=0.08) were observed, with maximum point at 11-12% of restriction. No effect (P>0.1) on percentage of M. dorsi fibers SO, FG and FOG was found. The meat from 128-kg-BW pig showed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Color; Fatty acid profile; Muscle fiber; Nutritional value; Tenderness.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500012
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Quality of aged meat of young bulls fed crude glycerin associated with different roughage sources Animal Sciences
Machado, Mirela; Lage, Josiane Fonseca; Ribeiro, Andressa Ferreira; Simonetti, Lais Regina; Oliveira, Emanuel Almeida; Berchielli, Telma Teresinha.
This trial aimed to evaluate the inclusion of 10%  of crude glycerin associated with roughage sources on the quality of meat aged for 1, 7 or 14 days post mortem of Nellore young bulls. Thirty feedlot animals (n = 10) with initial body weight of 416.70 ± 24.74 kg and 18 months of age were assigned to three treatments: corn silage (CS), sugar cane (SC) and sugar cane bagasse (CB), using a completely randomized design. After 85 days of feeding, animals were slaughtered with 554.51 ± 38.51 kg. Samples of longissimus muscle were collected, after carcass chilling, and vacuum-packed. Diets influenced pH, meat color and subcutaneous fat (SF) (p > 0.05). Animals fed CS showed higher values of b* in SF (p < 0.05). Differences were not found in the meat fatty...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Zootecnia Produção Animal beef cattle; Sugar cane bagasse; Fatty acids; Glycerol; Tenderness.
Ano: 2015 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/25695
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Quality of aged shoulder from lambs fed with different oldman saltbush hay levels (Atriplex nummularia) Ciência Rural
Alvarenga,Tharcilla Isabella Rodrigues Costa; Borba,Hirasilva; Moreno,Greicy Mitzi Bezerra; Araújo,Gherman Garcia Leal de; Mello,Juliana Lolli Malagoli de; Dourado,Rita de Cássia; Barbosa,José Carlos; Souza,Pedro Alves de.
This study assessed the effects of different levels of oldman saltbush hay and ageing time on the physical characteristics of Santa Inês lamb meat. Sixty shoulders from 32 male lambs fed with 30, 40, 50 or 60% oldman saltbush hay for 60 days were vacuum-packaged and stored in a refrigerator at 0 ± 1°C for 0, 7 or 14 days of ageing. The shear force, cooking loss and water holding capacity were 3.06kgf cm-2, 37.28% and 76.71%, respectively, and there were no significant changed by studied factors (P>0.05)
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cooking loss; Tenderness; Ageing; Sheep.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782014001202252
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The association of CAPN1 316 marker genotypes with growth and meat quality traits of steers finished on pasture Genet. Mol. Biol.
Miquel,María C.; Villarreal,Edgardo; Mezzadra,Carlos; Melucci,Lilia; Soria,Liliana; Corva,Pablo; Schor,Alejandro.
The objective of this paper was to determine the association of a SNP in the µ-calpain gene at position 316 with growth and quality of meat traits of steers grown on pasture. Fifty-nine Brangus and 20 Angus steers were genotyped for CAPN1 316. Warner Bratzler shear force was measured in l. lumborum samples after a 7-day aging period. A multivariate analysis of variance was performed, including shear force (WBSF), final weight (FW), average daily gain (ADG), backfat thickness (BFT), average monthly fat thickness gain (AMFTG), rib-eye area (REA), and beef rib-eye depth (RED) as dependent variables. The CAPN1 316 genotype was statistically significant. Univariate analyses were done with these variables. The marker genotype was statistically significant (p...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Beef cattle; Tenderness; Growth; Molecular marker; Multivariate analysis.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300011
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U.S. Consumers' Willingness to Pay for Flavor and Tenderness in Steaks as Determined with an Experimental Auction AgEcon
Feuz, Dillon M.; Umberger, Wendy J.; Calkins, Chris R.; Sitz, Bethany M..
In a study of beef quality, consumers tasted steak samples and participated in an experimental auction to determine their willingness to pay. Steaks differed in marbling, tenderness, country of origin, and aging method. Marbling and tenderness had statistically significant impacts on consumers' palatability ratings for steaks. Tenderness significantly impacted consumers' willingness-to pay values. There appear to be threshold levels of marbling and tenderness, below which consumers discount steaks. Steaks from Australia were rated lower for overall acceptability, and bids were lower than for the U.S. steak samples. Dry-aging methods negatively impacted taste panel ratings and bids.
Tipo: Journal Article Palavras-chave: Beef; Country of origin; Experimental auctions; Marbling; Tenderness; Consumer/Household Economics.
Ano: 2004 URL: http://purl.umn.edu/30913
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การศึกษาเปรียบเทียบคุณภาพและสีของเนื้อระหว่างการเก็บรักษาซากสุกร Thai Agricultural
Julakorn Panatuk; Suthipong Uriyapongson; Chainarong Navanukraw; Berg, Eric P..
The objectives of this study were to determined the variation of quality and shelf life color of pork carcass as affected by leg shackling. Six Largewhite barrows (130 kg) were slaugtered and one side of hind leg was shackled before exsanguination. The carcasses were splitted to two sides (shackled vs non-shackled) and carcass pH at loin and ham were recorded every 1 h for 4 h postmortem. Pork carcasses were aged for 9 days and loin samples were collected to determine for shear force and shelf life color everyday for 7 days. The result showed that pH at loin (Longissimus dorsi muscle) and Ham (Gluteus medius muscle) were similar between shackled and non-shackled side (P>0.05). The shear force of loin samples were similar between treatments. The color...
Tipo: PhysicalObject Palavras-chave: Pigs; Largewhite variety; Pork; Leg shackling; Shelf life; Carcass quality; Color; Tenderness; Muscle pH; Shear force value; สุกร; พันธุ์ลาจน์ไวน์; เนื้อสุกร; การฆ่าสัตว์; การตรึงขา; อายุการเก็บรักษา; คุณภาพซากสัตว์; ลักษณะปรากฎของสี; ความเหนียวนุ่ม; ความเป็นกรด-ด่าง; แรงตัดผ่านเนื้อ.
Ano: 2013 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5780
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ความนุ่มของเนื้อไก่ประดู่หางดำเชียงใหม่ 1 ไก่ลูกผสมประดู่หางดำเชียงใหม่ 1 และไก่กระทง Thai Agricultural
Ungkanaporn Pongduang; Amnuay Leotaragul; Aphirak Pianmongkol; Prodepran Thakeow; Sanchai Jaturasitha.
This experiment was conducted to investigate the effect of lines and muscle types on tenderness of Pradu Hang Dam Chiang Mai 1 (Pradu Hang Dam), Pradu Hang Dam’s crossbred (crossbred) and broiler at 1.2-1.3 kg of body weight. There were 80 chickens for each line group (total 240) and were allotted in CRD. For breast and thigh muscle, the results showed that thawing loss of Pradu Hang Dam and its crossbred were higher than broiler (P<0.05). For thigh muscle, cooking loss of Pradu Hang Dam was the highest (P<0.05). For shear force value, Pradu Hang Dam and its crossbred were higher than broiler both in breast and thigh muscle (P<0.05). For collagen content analysis, the results showed that Pradu Hang Dam had the highest content of soluble collagen...
Tipo: PhysicalObject Palavras-chave: Thawing loss; Cooking loss; Shear force value; Chicken; Lines; Muscle; Tenderness; Broiler; การสูญเสียน้ำจากการละลาย; การสูญเสียน้ำจากการประกอบอาหาร; ค่าแรงตัดผ่านของเนื้อ; ไก่ประดู่หางดำ; เนื้อสัตว์; เนื้อไก่.
Ano: 2013 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5410
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